Last updated: 2021-05-05

Checks: 7 0

Knit directory: NRCRI_2021GS/

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Unstaged changes:
    Modified:   README.md

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These are the previous versions of the repository in which changes were made to the R Markdown (analysis/04-GetGBLUPs.Rmd) and HTML (docs/04-GetGBLUPs.html) files. If you’ve configured a remote Git repository (see ?wflow_git_remote), click on the hyperlinks in the table below to view the files as they were in that past version.

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Rmd 94ec811 wolfemd 2021-05-05 Completed prediction. Build site and push to GitHub / Cassavabase FTP server. Share to NRCRI.

Previous step

  1. Check prediction accuracy: Evaluate prediction accuracy with cross-validation.

Objective

Current Step

  1. Genomic prediction: Predict genomic BLUPs (GEBV and GETGV) for all selection candidates using all available data.

Set-up

cd /home/jj332_cas/marnin/NRCRI_2021GS/; 
export OMP_NUM_THREADS=56 # activate multithread OpenBLAS 
library(tidyverse); library(magrittr); 
source(here::here("code","gsFunctions.R"))
A<-readRDS(file=here::here("output","Kinship_A_NRCRI_2021May03.rds"))

# BLUPs from the 2 stage procedure
# (stage 1 of 2) 
blups<-readRDS(file=here::here("output","NRCRI_blupsForModelTraining_twostage_asreml_2021May03.rds")) %>% 
  select(Trait,blups) %>% 
  unnest(blups) %>% 
  select(-`std error`) %>% 
  filter(GID %in% rownames(A)) %>% 
  nest(TrainingData=-Trait)

Prediction

runGenomicPredictions()

cbsurobbins (112 cores; 512GB)

Model A

options(future.globals.maxSize= 1500*1024^2)
predModelA<-runGenomicPredictions(blups,modelType="A",grms=list(A=A),gid="GID",ncores=14)
saveRDS(predModelA,file = here::here("output","genomicPredictions_ModelA_twostage_NRCRI_2021May03.rds"))

Model ADE

D<-readRDS(file=here::here("output","Kinship_D_NRCRI_2021May03.rds"))
AD<-readRDS(file=here::here("output","Kinship_AD_NRCRI_2021May03.rds"))
options(future.globals.maxSize= 3000*1024^2)
predModelADE<-runGenomicPredictions(blups,modelType="ADE",grms=list(A=A,D=D,AD=AD),gid="GID",ncores=14)
saveRDS(predModelADE,file = here::here("output","genomicPredictions_ModelADE_twostage_NRCRI_2021May03.rds"))

Write GEBV/GETV to disk

rm(list=ls()); gc()
          used (Mb) gc trigger (Mb) limit (Mb) max used (Mb)
Ncells  728874 39.0    1331574 71.2         NA  1331574 71.2
Vcells 1338811 10.3    8388608 64.0      65536  2225076 17.0
library(tidyverse); library(magrittr);
predModelA<-readRDS(file = here::here("output","genomicPredictions_ModelA_twostage_NRCRI_2021May03.rds"))
predModelADE<-readRDS(file = here::here("output","genomicPredictions_ModelADE_twostage_NRCRI_2021May03.rds"))
traits<-c("CGM","CGMS1","CGMS2","MCMDS",
          "DM","DMsg","PLTHT","BRNHT1","HI",
          "logDYLD","logFYLD","logTOPYLD","logRTNO")
unique_gids<-predModelA %>%
  dplyr::select(genomicPredOut) %>%
  unnest(genomicPredOut) %>%
  select(-varcomps) %>%
  unnest(gblups) %$%
  GID %>%
  unique

c1a<-unique_gids %>%
  grep("c1a",.,value = T,ignore.case = T) %>%
  union(.,unique_gids %>%
          grep("^F",.,value = T,ignore.case = T) %>%
          grep("c1b",.,value = T,ignore.case = T,invert = T))
c1b<-unique_gids%>% grep("c1b",.,value = T,ignore.case = T)
c2a<-unique_gids %>%
  grep("C2a",.,value = T,ignore.case = T) %>%
  grep("NR17",.,value = T,ignore.case = T)
c2b<-unique_gids %>%
  grep("C2b",.,value = T,ignore.case = T) %>%
  .[!. %in% c(c1a,c1b,c2a)]
c3a<-unique_gids %>%
  grep("C3a",.,value = T,ignore.case = T) %>%
  .[!. %in% c(c1a,c1b,c2a,c2b)]
c3b<-unique_gids %>%
  grep("NR20C3",.,value = T,ignore.case = T) %>%
  .[!. %in% c(c1a,c1b,c2a,c2b,c3a)]
nrTP<-setdiff(unique_gids,unique(c(c1a,c1b,c2a,c2b,c3a,c3b)))
## Format and write GEBV
predModelA %>% 
  select(Trait,genomicPredOut) %>% 
  unnest(genomicPredOut) %>% 
  select(-varcomps) %>% 
  unnest(gblups) %>% 
  select(-GETGV,-contains("PEV")) %>%
  spread(Trait,GEBV) %>% 
  mutate(Group=case_when(GID %in% nrTP ~ "nrTP",
                         GID %in% c1a ~ "C1a",
                         GID %in% c1b ~ "C1b",
                         GID %in% c2a ~ "C2a",
                         GID %in% c2b ~ "C2b",
                         GID %in% c3a ~ "C3a",
                         GID %in% c3b ~ "C3b")) %>% 
  select(Group,GID,any_of(traits)) %>% 
  arrange(desc(Group)) %>% 
  write.csv(., file = here::here("output","GEBV_NRCRI_ModelA_2021May03.csv"), row.names = F)

## Format and write GETGV
predModelADE %>% 
  select(Trait,genomicPredOut) %>% 
  unnest(genomicPredOut) %>% 
  select(-varcomps) %>% 
  unnest(gblups) %>% 
  select(GID,Trait,GETGV) %>% 
  spread(Trait,GETGV) %>% 
  mutate(Group=case_when(GID %in% nrTP ~ "nrTP",
                         GID %in% c1a ~ "C1a",
                         GID %in% c1b ~ "C1b",
                         GID %in% c2a ~ "C2a",
                         GID %in% c2b ~ "C2b",
                         GID %in% c3a ~ "C3a",
                         GID %in% c3b ~ "C3b")) %>% 
  select(Group,GID,any_of(traits)) %>% 
  arrange(desc(Group)) %>% 
  write.csv(., file = here::here("output","GETGV_NRCRI_ModelADE_2021May03.csv"), row.names = F)

### Make a unified "tidy" long-form: 
predModelA %>% 
  select(Trait,genomicPredOut) %>% 
  unnest(genomicPredOut) %>% 
  select(-varcomps) %>% 
  unnest(gblups) %>% 
  select(-GETGV) %>% 
  full_join(predModelADE %>% 
              select(Trait,genomicPredOut) %>% 
              unnest(genomicPredOut) %>% 
              select(-varcomps) %>% 
              unnest(gblups) %>% 
              rename(GEBV_modelADE=GEBV,
                     PEV_modelADE=PEVa) %>% 
              select(-genomicPredOut)) %>% 
  mutate(Group=case_when(GID %in% nrTP ~ "nrTP",
                         GID %in% c1a ~ "C1a",
                         GID %in% c1b ~ "C1b",
                         GID %in% c2a ~ "C2a",
                         GID %in% c2b ~ "C2b",
                         GID %in% c3a ~ "C3a",
                         GID %in% c3b ~ "C3b")) %>% 
  relocate(Group,.before = GID) %>% 
  write.csv(., file = here::here("output","genomicPredictions_NRCRI_2021May03.csv"), row.names = F)

Results

See Results: Home for plots and summary tables.


sessionInfo()
R version 4.0.3 (2020-10-10)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Big Sur 10.16

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRblas.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] magrittr_2.0.1  forcats_0.5.1   stringr_1.4.0   dplyr_1.0.5    
 [5] purrr_0.3.4     readr_1.4.0     tidyr_1.1.3     tibble_3.1.1   
 [9] ggplot2_3.3.3   tidyverse_1.3.1 workflowr_1.6.2

loaded via a namespace (and not attached):
 [1] tidyselect_1.1.0  xfun_0.22         bslib_0.2.4       haven_2.4.0      
 [5] colorspace_2.0-0  vctrs_0.3.7       generics_0.1.0    htmltools_0.5.1.1
 [9] yaml_2.2.1        utf8_1.2.1        rlang_0.4.10      jquerylib_0.1.3  
[13] later_1.1.0.1     pillar_1.6.0      withr_2.4.2       glue_1.4.2       
[17] DBI_1.1.1         dbplyr_2.1.1      readxl_1.3.1      modelr_0.1.8     
[21] lifecycle_1.0.0   cellranger_1.1.0  munsell_0.5.0     gtable_0.3.0     
[25] rvest_1.0.0       evaluate_0.14     knitr_1.32        httpuv_1.5.5     
[29] fansi_0.4.2       broom_0.7.6       Rcpp_1.0.6        promises_1.2.0.1 
[33] backports_1.2.1   scales_1.1.1      jsonlite_1.7.2    fs_1.5.0         
[37] hms_1.0.0         digest_0.6.27     stringi_1.5.3     rprojroot_2.0.2  
[41] grid_4.0.3        here_1.0.1        cli_2.4.0         tools_4.0.3      
[45] sass_0.3.1        crayon_1.4.1      whisker_0.4       pkgconfig_2.0.3  
[49] ellipsis_0.3.1    xml2_1.3.2        reprex_2.0.0      lubridate_1.7.10 
[53] rstudioapi_0.13   assertthat_0.2.1  rmarkdown_2.7     httr_1.4.2       
[57] R6_2.5.0          git2r_0.28.0      compiler_4.0.3